Information Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule mouse IgG.It also reacts with the light chains of other mouse immunoglobulins.No antibody was detected against non-immunoglobulin serum proteins.The antibody may cross-react with immunoglobulins from other species.

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Usage Physical State: Freeze-dried solid

Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.

Purity:The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 Stabilizer:15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free) Preservative:None(Warning:Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.) Suggested Working Concentration or Dilution Range:

1:500 - 1:5,000 for immunohisto/cytochemistry 1:5,000 - 1:100,000 for ELISA and Western blotting with chromogenic substrates 1:10,000 - 1:200,000 for Western blotting with ECL substratesDilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

Conjugate

Horseradish Peroxidase

Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.

Images & References

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This product is for in vitro research use only. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.

IMGENEX印度Pvt。Ltd.是印度东部地区最重要的生物技术公司之一，由Sujay Singh博士于2004年在布巴内斯瓦尔成立。IMGENEX印度公司已在Infocity大楼内建立了最先进的研发设施。IMGENEX印度公司的研发部门于2005年由当时的印度荣誉主席APJ Abdul Kalam博士成立，并获得了政府科学技术部科学与工业研究部（DSIR）的认可。印度。IMGENEX印度的研发部门拥有分子生物学，重组蛋白生产，细胞培养，抗体开发和其他下游工艺的所有现代化设备。

 

IMGENEX印度的主要目标是为特权较低的患者提供负担得起的医疗保健。多年来，IMGENEX印度公司已获得生物技术部（DBT）的几笔赠款，其中包括SIBRI赠款，用于使用基于纳米技术的药物递送开发用于骨质疏松症的药物。它处于早期开发阶段。除了获得BIRAC的资助外，我们还帮助我们在CHO细胞中开发了高产量的哺乳动物表达系统，该系统能够产生每升生物仿制药曲妥珠单抗/赫赛汀3-4 g。计划中的其他生物仿制药包括Adalimumab（humira），Bevacizuma（avastin），Ustekinumab（stellara）和Nivolumab（opdivo）。

 

IMGENEX印度与印度医学研究理事会（ICMR）的一个部门国家免疫血液学研究所合作，正在开发首个用于检测β地中海贫血的本地诊断试剂盒，该试剂盒可通过ICMR很快获得。

 

IMGENEX印度公司还根据授予ICAR-印度农业研究所的CRS计划，开发和验证针对影响园艺作物的植物病毒的血清学诊断试剂和试剂盒。

 

IMGENEX印度公司在2011年被新加坡《生物频谱》杂志评为“亚太地区新兴公司”，并被班加罗尔生物技术公司评为印度生物行业2015年度新兴公司。

 

目前，我们正在奥里萨邦布巴内斯瓦尔建设14,000平方英尺的GMP设施，以使用一次性生物反应器生产重组治疗性抗体和蛋白质。